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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
Standard Clinical Chemistry Reagents For Cholesterol Cl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standard clinical chemistry reagents for cholesterol cl - by Bioz Stars, 2026-07
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
Hba1c Measurement According To The Federation Of Clinical Chemistry And Medicine (Ifcc) Standard, supplied by International Federation of Clinical Chemistry and Laboratory Medicine, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
Who International Federation For Clinical Chemistry And Medicine (Ifcc) Standard Srm2b, supplied by International Federation of Clinical Chemistry and Laboratory Medicine, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), <t>cholesterol</t> (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.
Hba1c Standard From The Federation Of Clinical Chemistry And Medicine (Ifcc), supplied by International Federation of Clinical Chemistry and Laboratory Medicine, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), cholesterol (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.

Journal: bioRxiv

Article Title: Calpain-4 Knockdown Modulates Cholesterol Metabolism and LXRα Nuclear Localization in Alcohol-Related Liver Disease

doi: 10.1101/2025.10.09.681083

Figure Lengend Snippet: Panel A: Hematoxylin and eosin (H&E; 20×) shows general histology. Panel B: Total levels of free fatty acid (FFA), triglycerides (TG), cholesterol (CL) concentrations and β-hydroxybutyrate (βHB) were determined biochemically. Panel C: Small lipid droplets were scored in HE-stained sections to evaluate the degree of microvesicular steatosis. Tissue scores were assigned based on the percentage of microvesicular steatosis observed in a single field as follows: Score 0: 0%; Score 1: 1–25%; Score 2: 26–50%; Score 3: 51–75%; Score 4: >75%.

Article Snippet: Hepatic lipids were determined using standard clinical chemistry reagents for cholesterol (CL) and triglycerides (TG) (Infinity, Thermo Fisher Scientific, Waltham, MA), for free fatty acids (FFA) (Cell Biolabs, Inc., San Diego, CA) and β-hydroxybutyrate (βHB) (Colorimetric, Cell Biolabs, Inc., San Diego, CA) (See supplemental Table1).

Techniques: Staining